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CCR4 Antibody
SKU: orb412962
Description
Research Area
Cardiovascular Research, Immunology & Inflammation
Images & Validation
−Item 1 of 1
| Tested Applications | WB |
|---|---|
| Dilution range | Western blot,0.1-0.5μg/ml |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Immunogen | A synthetic peptide corresponding to a sequence at the C-terminus of human CCR4, which shares 93.3% amino acid (aa) sequence identity with both mouse and rat CCR4. |
| Target | C-C chemokine receptor type 4 |
| Molecular Weight | 41 kDa |
| Purification | Immunogen affinity purified. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.05mg NaN3. |
| Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
| Disclaimer | For research use only |
Alternative Names
−C-C CKR-4; CC-CKR-4; CCR-4; CCR4; K5-5; CD194; CCR4; CMKBR4; CKR4; ChemR13; HGCN:14099
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Western blot analysis of CCR4 using anti-CCR4 antibody. Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50 ug of sample under reducing conditions. Lane 1: rat thymus tissue lysates, Lane 2: mouse thymus tissue lysates, Lane 3: mouse NIH3T3 whole cell lysates. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CCR4 antigen affinity purified polyclonal antibody at 0.5 µg/mL overnight at 4°C, then washed with TBS-0.1% Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CCR4 at approximately 41KD. The expected band size for CCR4 is at 41KD.
Quick Database Links
Gene Symbol
C-C chemokine receptor type 4
UniProt
UniProt Details
− No UniProt data available
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CCR4 Antibody (orb412962)
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