Anti-CCR5 (phosphoserine 337) [V14/2]

SKU: orb758955

Description

Mouse monoclonal antibody to CCR5 (phosphoserine 337)

Research Area

Immunology & Inflammation, Protein Biochemistry

Images & Validation

−

Item 1 of 3

Tested Applications	ELISA, FC, IF, IP, WB
Reactivity	Human

Key Properties

−

Host	Mouse
Clonality	Monoclonal
Isotype	IgG2b
Clone No.	V14/2
Immunogen	This antibody was raised by immunizing mice with a synthetic phospho peptide corresponding to the C-Terminal region of human CCR5 conjugated to BSA.
Target	CCR5 (phosphoserine 337)
Purity	Purified
Conjugation	Unconjugated

Storage & Handling

−

Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	PBS with 0.02% Proclin 300.
Concentration	1 mg/ml
Disclaimer	For research use only

Alternative Names

−

CD195; C-C chemokine receptor type 5; C-C CKR-5; CC-CKR-5; CCR-5; CHEMR13; HIV-1 fusion coreceptor; p337; pS337; Ser-337; Ser337

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Flow cytometry using the anti-CCR5 (phosphoserine 349) antibody E11/19. U937 cells were fixed using 2% PFA and stained with anti-unknown specificity antibody (orb256458; isotype control, black line) or the rabbit IgG1 version of E11/19 (orb758954, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of fixed U937 cells with anti-CCR5 (phosphoserine 337) antibody V14/2. Immunofluorescence analysis of paraformaldehyde fixed U937 cells on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of V14/2 (orb758954) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb758954, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (orb256458) followed by staining with Alexa Fluor® 488 secondary antibody.

Western Blot using anti-CCR5 (phosphoserine 337) antibody V14/2 K562 cell lysate (35 µg protein in RIPA buffer) was resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of V14/2 (orb758954) at 2 µg/ml before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.

Quick Database Links

Gene Symbol

CCR5 (phosphoserine 337)

UniProt

P51681

UniProt Details

−

No UniProt data available

Documents Download

Datasheet

Product Information

Download

Request a Document

Protocol Information

Western Blot (IB, immunoblot)

View Protocol

Flow Cytometry

View Protocol

Immunofluorescence

View Protocol

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

View Protocol

Immunoprecipitation

View Protocol

Find More Protocols