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CD22 Antibody
SKU: orb689339
Featured
Description
Images & Validation
−Item 1 of 5
| Tested Applications | ELISA, FC |
|---|---|
| Dilution Range | Flow Cyt 1:100 |
| Reactivity | Human |
Key Properties
−| Antibody Type | Recombinant Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | Rabbit IgG |
| Clone No. | DM13 |
| Target | CD22 |
| Purification | Purified from cell culture supernatant by affinity chromatography |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−SIGLEC-2, SIGLEC2
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Expi 293 cell line transfected with irrelevant protein (left) and human CD22 (right) were surface stained with Rabbit anti- CD22 monoclonal antibody 1 μg/ml (clone: DM13) followed by Alexa 488-conjugated anti-rabbit IgG secondary antibody.
Flow cytometry data of serially titrated Rabbit anti-CD22 monoclonal antibody (clone: DM13) on Raji cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.
Affinity ranking of different Rabbit anti-CD22 mAb clones by titration of different concentration onto Raji cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.
Phylogenetic analysis of amino acid sequence of different Rabbit Anti-CD22 mAb clones. A) Heavy chain and B) Light chain.
Flow cytometry analysis of antigen binding of rabbit anti-human CD22 mAb (orb689339). (A) orb689339 does not bind to Jurkat cells that do not express CD22. (B) A clear peak shift of orb689339 was seen compared to the control when incubated with CD22-expressing Raji cells, indicating strong binding of orb689339 to CD22. Antibodies were incubated at 10 μg/mL.
Quick Database Links
Gene Symbol
CD22
UniProt
UniProt Details
− No UniProt data available
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Datasheet
Product Information
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Protocol Information
FC
Flow Cytometry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
CD22 Antibody (orb689339)
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