Anti-CD257 (BAFF) [Tabalumab]

SKU: orb1152486

Description

Rabbit monoclonal antibody to CD257

Research Area

Immunology & Inflammation

Images & Validation

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Item 1 of 1

Tested Applications	ELISA, FC, Functional Studies, IF, IP, WB
Reactivity	Human, Monkey, Rabbit

Key Properties

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Host	Human
Clonality	Monoclonal
Isotype	Human IgG
Clone No.	Tabalumab
Immunogen	Monoclonal antibodies were generated by immunizing human immunoglobulin transgenic mice with soluble human BAFF (amino acids 133–285). Eight mice producing serum antibody titers to human BAFF were injected intravenously with 10 µg human BAFF in phosphate buffered saline. The spleen was harvested 3 days later from each mouse and fused with myeloma cells. 20 Hybridomas were tested for specific binding to human BAFF and to make sure they were expressing human immunoglobulin heavy and light chains by enzyme-linked immunosorbent assay (ELISA). The hybridoma producing the antibody that eventually became tabalumab was a human IgG1 isotype. The antibody was then engineered to a human IgG4 isotype for complete neutralization of BAFF without depletion of BAFF-expressing cells.
Target	CD257 (BAFF)
Purity	Purified
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	PBS with 0.02% Proclin 300.
Concentration	1 mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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TNFSF20; B-cell activating factor; Tumor Necrosis Factor Superfamily Member 13b; BAFF; Tumor Necrosis Factor (Ligand) Superfamily, Member 13b; Dendritic Cell-Derived TNF-Like Molecule; TALL-1; TALL1; ZTNF4; BLYS; Tumor Necrosis Factor (Ligand) Superfamily, Member 20; Tumor Necrosis Factor Ligand Superfamily Member 13B; TNF- And APOL-Related Leukocyte Expressed Ligand 1; TNF And ApoL-Related Leukocyte Expressed Ligand 1; Tumor Necrosis Factor-Like Protein ZTNF4; TNF Homolog That Activates Apoptosis; Tumor Necrosis Factor Ligand 7A; ApoL Related Ligand TALL-1; B-Lymphocyte Stimulator; B Lymphocyte Stimulator; CD257 Antigen; Delta4 BAFF; Delta BAFF; TNLG7A; THANK; DTL; LY 2127399; LY2127399

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Immunofluorescence staining of human peripheral blood monocytes with anti-CD257 (BAFF) Tabalumab. Immunofluorescence analysis of paraformaldehyde fixed human peripheral blood monocytes on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of Tabalumab (orb1152486) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb1152486, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (orb256458) followed by staining with Alexa Fluor® 488 secondary antibody.

Quick Database Links

Gene Symbol

CD257 (BAFF)

UniProt

Q9Y275

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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Flow Cytometry

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Immunofluorescence

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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Immunoprecipitation

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