Anti-CD27 [LG.3A10]

Binding curve of anti-CD27 antibody LG.3A10 (orb613905) to mouse CD27-Fc fusion protein ELISA Plate coated with mouse CD27-Fc fusion protein at a concentration of 5 µg/ml. A 3-fold serial dilution from 10000 to 0.1 ng/ml was performed using orb613905. For detection, a 1:4000 dilution of HRP-labelled goat anti-rabbit antibody was used.

Immunofluorescence staining of fixed mouse splenocytes with anti-CD27 antibody LG.3A10. Immunofluorescence analysis of paraformaldehyde fixed mouse (Mus musculus) splenocytes on Shi-fix™ coverslips, permeabilized with 0.15% Triton stained with the chimeric rabbit version of LG.3A10 (orb613905) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (1 µg/ml), showing membrane and some cytoplasmic staining in a subset of cells. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb613905, DAPI, merged channels and an isotype control. The isotype control was stained with an anti-Fluorescein antibody followed by Alexa Fluor® 488 secondary antibody.