Anti-CD27 [RM27-3E5]

SKU: orb613954

Description

Rabbit monoclonal antibody to CD27

Research Area

Immunology & Inflammation

Images & Validation

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Item 1 of 2

Tested Applications	FC, IP
Reactivity	Mouse

Key Properties

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Host	Rat
Clonality	Monoclonal
Isotype	Rat IgG
Clone No.	RM27-3E5
Immunogen	This antibody was raised by immunising mouse with mouse CD27-human IgG1 Fc fusion protein. Isolated popliteal lymph node cells were then fused with P3U1 myeloma cells to produce stable hybridomas.
Target	CD27
Purity	Purified
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	PBS with 0.02% Proclin 300.
Concentration	1 mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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TNFRS7; CD27 antigen; CD27L receptor; T-cell activation antigen CD27; Tumor necrosis factor receptor superfamily member 7

Quality Guarantee

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ELISA of anti-CD27 antibody on CD27-Fc fusion protein, with a CD70 (CD27-L) competition assay. Binding curves of the rabbit IgG chimeric version of the anti-CD27 (Interleukin-2 receptor subunit alpha) antibody RM27-3E5 (orb613954; red line) and isotype control (anti-Fluorescein antibody orb256390 4-4-20 (enhanced); blue line) to an ELISA plate coated with CD27-Fc fusion protein at a concentration of 5 µg/ml. A 3-fold serial dilution from 10 mg/mL to 0.17 ng/ml of orb613954 was used.

ELISA of anti-CD27 antibody on CD27-Fc fusion protein. Binding curves of the human chimeric version of the anti-CD27 (Interleukin-2 receptor subunit alpha) antibody RM27-3E5 (red line) and isotype control (anti-Fluorescein antibody orb256390 4-4-20 (enhanced); blue line) to an ELISA plate coated with CD27-Fc fusion protein at a concentration of 5 µg/ml.

Quick Database Links

Gene Symbol

CD27

UniProt

P41272

UniProt Details

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No UniProt data available

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Protocol Information

Flow Cytometry

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Immunoprecipitation

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