CD282 Mouse Monoclonal Antibody

SKU: orb248906

Description

The CD282 Antibody is suitable for FC, IF. It is a Monoclonal, Unconjugated antibody which raised against Native purified human CD282. Purification: The antibody was purified by affinity chromatography.

Research Area

Immunology & Inflammation

Images & Validation

−

Item 1 of 1

Tested Applications	FC, IF
Dilution Range	IF/IC (1/50 - 1/200), FC (1/500 - 1/2000)
Reactivity	Human
Application Notes	1. Take 100 μl peripheral blood anticoagulated by EDTA and add to the bottom of 5 ml tube. 2. Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood; incubate for 30 minutes at room temperature. 3. Add 2 ml RBC lysis buffer; incubate for 10 minutes after mixing; dissolve red blood cells. 4. Sample tube is set to 1000 rpm centrifugation for 5 minutes; discard the supernatant. 5. Add 2 ml PBS wash buffer to resuspend the cells; then 1000 rpm centrifugation for 5 minutes; discard the supernatant. 6. Add appropriate amount of fluorescent-labeled anti-mouse IgGs and incubate for 20 minutes away from light at room temperature. 7. Add 2 ml PBS wash buffer to resuspend the cells; then 1000 rpm centrifugation for 5 minutes; discard the supernatant. 8. Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 °C then measured). Read more...

Key Properties

−

Antibody Type	Primary Antibody
Host	Mouse
Clonality	Monoclonal
Isotype	Mouse IgG2b
Clone No.	BV4X7
Immunogen	Native purified human CD282.
Target	TLR2
Purification	The antibody was purified by affinity chromatography.
Conjugation	Unconjugated

Storage & Handling

−

Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	PBS, pH 7.3, and 0.02% sodium azide.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

−

TIL4; Toll-like receptor 2; Toll/interleukin-1 receptor-like protein 4; CD antigen CD282

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Flow cytometric analysis of human peripheral blood monocytes using Anti-CD282 Antibody, followed by anti-mouse IgG PE.

Quick Database Links

Gene Symbol

TLR2

UniProt

O60603

NCBI Gene ID

7097

UniProt Details

−

No UniProt data available

NCBI Gene Details

−

No NCBI Gene data available

Documents Download

Datasheet

Product Information

Download

Request a Document

Protocol Information

Flow Cytometry

View Protocol

Immunofluorescence

View Protocol

Find More Protocols