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CD47 Antibody
SKU: orb1805751
Description
Images & Validation
−Item 1 of 6
| Tested Applications | ELISA, FA, FACS, In vivo |
|---|---|
| Reactivity | Human |
Key Properties
−| Clonality | Monoclonal |
|---|---|
| Target | CD47 |
| Source/Expression System | CHO |
| Molecular Weight | 145.58 kDa |
| Purity | >95% |
| Purification | Protein A |
| Endotoxin | <0.001 EU/ug |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | 100 mM Proline/Acetic acid 20mM Arginine pH 5.0 |
| Concentration | Batch dependent |
| Disclaimer | For research use only |
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Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].
Anti-CD47 Reference Antibody (lemzoparlimab) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Anti-CD47 Reference Antibody (lemzoparlimab)is more than 100% ;determined by SEC-HPLC.
Immobilized Recomvinant human CD47(c His) at1.5 μg/mL can bind Anti-CD47 Reference Antibody (lemzoparlimab);EC50=0.0163μg/mL.
PANC-1 cells were stained with Anti-CD47 Reference Antibody (lemzoparlimab) and negative control protein respectively; washed and then followed by PE and analyzed with FACS; EC317=0.265 ug/mL.
Anti-CD47 Reference Antibody (lemzoparlimab) FACS Blocking was evaluated using CCRF-CEM. The IC50 was approximately 0.4557 ug/mL.
The phagocytosis ratio lemzoparlimab by CCRF-CEM increased with the increase of antibody concentration; and the phagocytosis Rate (%) reached 43%.
Quick Database Links
Gene Symbol
CD47
UniProt
UniProt Details
− No UniProt data available
Documents Download
Datasheet
Product Information
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Protocol Information
FACS
Fluorescence-Activated Cell Sorting (FC, Flow cytometry)
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
CD47 Antibody (orb1805751)
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