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CD47 Biosimilar Antibody
SKU: orb1173629
Featured
Description
Images & Validation
−Item 1 of 3
| Tested Applications | ELISA, FC |
|---|---|
| Dilution range | ELISA 1:5000-10000; Flow Cyt 1:100 |
| Reactivity | Human |
Key Properties
−| Antibody Type | Biosimilar Antibody |
|---|---|
| Host | Human Humanized |
| Clonality | Monoclonal |
| Isotype | Human IgG4 - Kappa |
| Target | CD47 |
| Purification | Purified from cell culture supernatant by affinity chromatography |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Powder |
| Disclaimer | For research use only |
Alternative Names
−CD47;MER6;IAP
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ELISA plate pre-coated by 1 μg/mL (100 μL/well) Human CD47 protein, mFc-His Tag orb689369 can bind Anti-CD47 Neutralizing antibody (orb1173629) in a linear range of 3.2-400 ng/ml.
Jurkat cell line were surface stained with anti-CD47 (orb1173629) (Blue histogram) and isotype control (Red histogram) 1 μg/ml followed by Alexa 488-conjugated Goat anti-Human IgG secondary antibody.
Flow cytometry analysis of antigen binding of anti-human CD47 mAb (orb1173629). (A) orb1173629 does not bind to CHO-S cells that do not express CD47. (B) A clear peak shift of orb1173629 was seen compared to the control when incubated with CD47-expressing 8226 cells, indicating strong binding of orb1173629 to CD47. Antibodies were incubated at 5 μg/mL.
Quick Database Links
Gene Symbol
CD47
UniProt
UniProt Details
− No UniProt data available
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Datasheet
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Protocol Information
FC
Flow Cytometry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
CD47 Biosimilar Antibody (orb1173629)
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