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Anti-CD70 [TAN1-6]
SKU: orb758814
Description
Research Area
Immunology & Inflammation
Images & Validation
−Item 1 of 2
| Tested Applications | Blocking, FC, IF, WB |
|---|---|
| Reactivity | Mouse |
Key Properties
−| Host | Rat |
|---|---|
| Clonality | Monoclonal |
| Isotype | IgG2a |
| Clone No. | TAN1-6 |
| Immunogen | This antibody was raised by immunising LOU rats with soluble recombinant CD70:Fc fusion protein |
| Target | CD70 |
| Purity | Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | PBS with 0.02% Proclin 300. |
| Concentration | 1 mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−TNFSF7; CD27 ligand; CD27-L; Cd27l; CD27LG; CD70 antigen; Ki-24 antigen; Tnfsf7; tumor necrosis factor (ligand) superfamily, member 7; Tumor necrosis factor ligand superfamily member 7
Similar Products
−- Item 1 of 2
![Anti-CD70 [TAN1-6]](/images/pub/media/catalog/product/NewWebsite/35/orb758813_1.png)
![Anti-CD70 [TAN1-6]](/images/pub/media/catalog/product/NewWebsite/35/orb758813_2.png)
Quality Guarantee
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![Anti-CD70 [TAN1-6]](/images/pub/media/catalog/product/NewWebsite/35/orb758814_1.png)
Flow cytometry using the Anti-CD70 antibody TAN1-6. Paraformaldehyde fixed mouse splenocytes permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (orb256458; isotype control, black line) or the rabbit IgG version of TAN1-6 (orb758813, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.
![Anti-CD70 [TAN1-6]](/images/pub/media/catalog/product/NewWebsite/35/orb758814_2.png)
Immunofluorescence staining of fixed RAW264.7 cells with anti-CD70 antibody TAN1-6. Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of TAN1-6 (orb758813) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb758813, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (orb256449) followed by staining with Alexa Fluor® 488 secondary antibody
Quick Database Links
Gene Symbol
CD70
UniProt
UniProt Details
− No UniProt data available
Documents Download
Datasheet
Product Information
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Protocol Information
WB
Western Blot (IB, immunoblot)
FC
Flow Cytometry
IF
Immunofluorescence
Anti-CD70 [TAN1-6] (orb758814)
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