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CDK1 Antibody
SKU: orb44473
Description
Research Area
Neuroscience
Images & Validation
−Item 1 of 2
| Tested Applications | ICC, IHC-P, IP, WB |
|---|---|
| Reactivity | Bovine, Human, Primate |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Clonality | Monoclonal |
| Isotype | Mouse IgG2a |
| Clone No. | POH-1 |
| Immunogen | Bacterially expressed full-length human Cdk1 protein |
| Target | CDK1 |
| Purification | Purified by protein-A affinity chromatography. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Concentration | 1 mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−p34Cdc2, p34 protein kinase, CDK1, CDC2, CDC28A
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Immunohistochemistry staining of human thymus (paraffin sections) using anti-Cdk1 (clone POH-1).
Western blotting analysis of human Cdk1 using mouse monoclonal antibody POH-1 on lysates of HeLa, MOLT-4, and Jurkat cells under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse anti-Cdk1 monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. A specific band was detected for Cdk1 protein at approximately 32 kDa.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
ICC
Immunocytochemistry
IP
Immunoprecipitation
CDK1 Antibody (orb44473)
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