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Anti-CEA [EB-022]
Description
Research Area
Images & Validation
−| Tested Applications | ELISA |
|---|---|
| Reactivity | Human |
Key Properties
−| Host | Mouse |
|---|---|
| Clonality | Monoclonal |
| Isotype | Mouse IgG |
| Clone No. | EB-022 |
| Immunogen | This antibody was generated by immunising BALB/c mice. Splenocytes from immunised mice were obtained and fused with myeloma cells to generate a hybridoma. |
| Target | CEA |
| Purity | Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | PBS with 0.02% Proclin 300. |
| Concentration | 1 mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
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![Anti-CEA [EB-022]](/images/pub/media/catalog/product/NewWebsite/35/orb1463371_1.png)
Immunofluorescence staining of MCF7 cells with anti-CEA EB-022. Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells stained with the chimeric rabbit IgG version of EB-022 (orb1463371) (1:1000 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1500 dilution), showing nuclear staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb1463371, DAPI, merged channels and an isotype control. The isotype control was anti-fluorescein antibody (orb256390) followed by staining with Alexa Fluor® 488 secondary antibody.
![Anti-CEA [EB-022]](/images/pub/media/catalog/product/NewWebsite/35/orb1463371_2.png)
Western Blot using anti-CEA antibody EB-022. Human colorectal carcinoma (0.01 µg/ml) tissue lysate (35 µg protein in RIPA buffer) was resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of EB-022 (orb1463371) before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.
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Anti-CEA [EB-022] (orb1463371)
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