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Anti-CEA [EB-022]

SKU: orb1463372

Description

Mouse monoclonal antibody to CEA

Research Area

Cancer Biology, Cell Biology

Images & Validation

Tested ApplicationsELISA
ReactivityHuman

Key Properties

HostMouse
ClonalityMonoclonal
IsotypeIgG1
Clone No.EB-022
ImmunogenThis antibody was generated by immunising BALB/c mice. Splenocytes from immunised mice were obtained and fused with myeloma cells to generate a hybridoma.
TargetCEA
PurityPurified
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesPBS with 0.02% Proclin 300.
Concentration1 mg/ml
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

Carcinoembryonic antigen; Carcinoembryonic antigen-related cell adhesion molecule 5; CEACAM5; Cluster of Differentiation 66e; CD66e

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  • Anti-CEA [EB-022] [orb1463371]

    ELISA

    Human

    Mouse

    Monoclonal

    Unconjugated

    0.2 mg
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Anti-CEA [EB-022]

Immunofluorescence staining of MCF7 cells with anti-CEA EB-022. Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells stained with the chimeric rabbit IgG version of EB-022 (orb1463371) (1:1000 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1500 dilution), showing nuclear staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb1463371, DAPI, merged channels and an isotype control. The isotype control was anti-fluorescein antibody (orb256390) followed by staining with Alexa Fluor® 488 secondary antibody.

Anti-CEA [EB-022]

Western Blot using anti-CEA antibody EB-022. Human colorectal carcinoma (0.01 µg/ml) tissue lysate (35 µg protein in RIPA buffer) was resolved on a SDS PAGE gel and blots were probed with the chimeric rabbit version of EB-022 (orb1463371) before detection using an anti-rabbit secondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence.

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Protocol Information

ELISA
Enzyme-linked Immunosorbent Assay (EIA)
View Protocol

Anti-CEA [EB-022] (orb1463372)

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Available Sizes

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0.2 mg
¥ 7,800.00
DispatchUsually dispatched within 5-10 working days
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