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CLIP2 Antibody
SKU: orb214737
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Description
Images & Validation
−Item 1 of 2
| Tested Applications | IF, WB |
|---|---|
| Dilution range | WB: 1-500-1-1000 |
| Reactivity | Human, Monkey, Mouse, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the C-term region of human CLIP2. The exact sequence is proprietary. |
| Purification | The antibody was purified by immunogen affinity chromatography. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
| Disclaimer | For research use only |
Alternative Names
−CYLN2; KIAA0291; WBSCR3; WBSCR4; WSCR4; CAP-Gly domain-containing linker protein 2; Cytoplasmic linker protein 115; CLIP-115; Cytoplasmic linker protein 2; Williams-Beuren syndrome chromosomal region 3 protein; Williams-Beuren syndrome chromosomal region 4 protein
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CLIP2 Rabbit Polyclonal Antibody [orb156512]
ELISA, ICC, IF, IHC-Fr, IHC-P
Bovine, Canine, Equine, Human, Mouse, Sheep
Rat
Rabbit
Polyclonal
Unconjugated
50 μl, 100 μl, 200 μl - Item 1 of 1
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Western blot analysis of CLIP2 expression in AML12 (A), PC12 (B), A549 (C), LO2 (D), Hela (E) whole cell lysates. (Predicted band size: 115 kD; Observed band size: 130 kD)
Immunofluorescent analysis of Vimentin staining in CLIP2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
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Protocol Information
WB
Western Blot (IB, immunoblot)
IF
Immunofluorescence
CLIP2 Antibody (orb214737)
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