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Collagen 6 alpha 2 Rabbit Polyclonal Antibody
SKU: orb213763
Featured
Description
Research Area
Epigenetics
Images & Validation
−Item 1 of 5
| Tested Applications | IF, IHC, WB |
|---|---|
| Dilution Range | WB: 1-500-1-1000, IHC-P: 1-100-1-200, IF/ICC: 1-100-1-500, IP: 1-10-1-100 |
| Reactivity | Human, Monkey, Mouse, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Immunogen | KLH-conjugated synthetic peptide encompassing a sequence within the center region of human Collagen 6 alpha 2. The exact sequence is proprietary. |
| Target | COL6A2 |
| Molecular Weight | 108 kDa |
| Purification | The antibody was purified by immunogen affinity chromatography. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Collagen alpha-2(VI) chain
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200 μl, 100 μl, 50 μl- Item 1 of 1
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Immunofluorescent analysis of Collagen 6 alpha 2 staining in HT29 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
Immunohistochemical analysis of paraffin-embedded human liver cancer using Collagen 6 alpha 2 Antibody orb213763 with dilution at 1:100.
Immunohistochemical analysis of paraffin-embedded human lung cancer using Collagen 6 alpha 2 Antibody orb213763 with dilution at 1:100.
Immunohistochemical analysis of paraffin-embedded rat stomach using Collagen 6 alpha 2 Antibody orb213763 with dilution at 1:100.
Western blot analysis of Hela (1) using Collagen 6 alpha 2 Antibody orb213763 with dilution at 1:1000.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
IF
Immunofluorescence
Collagen 6 alpha 2 Rabbit Polyclonal Antibody (orb213763)
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