Cytokeratin 19 Antibody (FITC)

SKU: orb154471

Description

Mouse monoclonal antibody conjugated to FITC which recognizes Cytokeratin 19 that belongs to the intermediate filament family. It expressed in a defined zone of basal keratinocytes in the deep outer root sheath of hair follicles. It involved in the organization of myofibers. Cytokeratin 19 is a specific marker of moderate to severe dysplasia and carcinoma in situ in oral cavity squamous epithelium, and measurement of Cytokeratin 19 may be a useful marker in diagnosing hepatoma.

Research Area

Epigenetics, Infectious Diseases

Images & Validation

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Tested Applications	FC
Reactivity	Human
Application Notes	Flow cytometry: Recommended dilution: 1-5 μg/ml. Intracellular staining.

Key Properties

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Antibody Type	Primary Antibody
Clonality	Monoclonal
Isotype	Mouse IgG2a
Clone No.	A53-B/A2
Immunogen	MCF-7 human breast adenocarcinoma cell line
Target	Cytokeratin 19
Purification	Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Conjugation	FITC

Storage & Handling

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Storage	Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Buffer/Preservatives	Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Concentration	1 mg/ml
Disclaimer	For research use only

Alternative Names

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K19, CK19, CYK19, KRT19, K1CS

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Separation of MCF-7 cells stained using anti-Cytokeratin 19 (A53-B/A2) FITC antibody (concentration in sample 3 µg/ml, red-filled) from MCF-7 cells stained using mouse IgG2b isotype control (MPC-11) FITC antibody (concentration in sample 3 µg/ml, same as anti-Cytokeratin 19 FITC antibody concentration, black-dashed) in flow cytometry analysis (surface staining).

Quick Database Links

Gene Symbol

Cytokeratin 19

UniProt

P08727

NCBI Gene ID

3880

UniProt Details

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No UniProt data available

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Flow Cytometry

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