Dkk1 Rabbit Polyclonal Antibody

SKU: orb1147778

Description

Anti-Dkk1 Antibody. Tested in ELISA, IHC applications. This antibody reacts with Mouse, Rat.

Research Area

Cardiovascular Research, Immunology & Inflammation, Protein Biochemistry, Stem Cell & Developmental Biology

Images & Validation

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Item 1 of 3

Tested Applications	ELISA, IHC
Dilution Range	Immunohistochemistry(Paraffin-embedded Section), 2-5 μg/ml, Mouse, Rat ELISA, 0.1-0.5 μg/ml, -
Reactivity	Mouse, Rat

Related Conjugates & Formulations

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Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Isotype	Rabbit IgG
Immunogen	E.coli-derived mouse Dkk1 recombinant protein (Position: N73-H272).
Target	Dickkopf-related protein 1
Molecular Weight	38 kDa
Purification	Immunogen affinity purified.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Lyophilized
Buffer/Preservatives	Each vial contains 4 mg Trehalose, 0.9 mg NaCl, 0.2 mg Na2HPO4.
Concentration	Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Dickkopf 1; Dickkopf related protein 1; DKK 1; DKK1; hDkk 1; SK

Quality Guarantee

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IHC analysis of Dkk1 using anti-Dkk1 antibody. Dkk1 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-Dkk1 Antibody overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.

IHC analysis of Dkk1 using anti-Dkk1 antibody. Dkk1 was detected in a paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-Dkk1 Antibody overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.

IHC analysis of Dkk1 using anti-Dkk1 antibody. Dkk1 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 µg/ml rabbit anti-Dkk1 Antibody overnight at 4°C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.

Quick Database Links

Gene Symbol

Dickkopf-related protein 1

UniProt

O54908

UniProt Details

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No UniProt data available

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Protocol Information

IHC

Immunohistochemistry

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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