EIF4G2 Rabbit Polyclonal Antibody

SKU: orb213886

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Description

The EIF4G2 Antibody is suitable for IF, IHC, WB. It is a Polyclonal, Unconjugated antibody which raised against KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human EIF4G2. The exact sequence is proprietary. Purification: The antibody was purified by immunogen affinity chromatography.

Research Area

Epigenetics

Images & Validation

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Item 1 of 5

Tested Applications	IF, IHC, WB
Dilution Range	WB: 1-500-1-1000, IHC-P: 1-100-1-200, IF/ICC: 1-100-1-500
Reactivity	Bovine, Human, Monkey, Mouse, Rabbit, Rat

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Immunogen	KLH-conjugated synthetic peptide encompassing a sequence within the N-term region of human EIF4G2. The exact sequence is proprietary.
Target	EIF4G2
Purification	The antibody was purified by immunogen affinity chromatography.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Buffer/Preservatives	0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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DAP5; Eukaryotic translation initiation factor 4 gamma 2; eIF-4-gamma 2; eIF-4G 2; eIF4G 2; Death-associated protein 5; DAP-5; p97

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Immunofluorescent analysis of EIF4G2 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).

Western blot analysis of mouse heart (1), rat heart (2) using EIF4G2 Rabbit Polyclonal Antibody orb213886 with dilution at 1:1000.

Immunohistochemical analysis of paraffin-embedded human liver cancer using EIF4G2 Rabbit Polyclonal Antibody orb213886 with dilution at 1:100.

Immunohistochemical analysis of paraffin-embedded mouse spleen using EIF4G2 Rabbit Polyclonal Antibody orb213886 with dilution at 1:100.

Immunohistochemical analysis of paraffin-embedded rat stomach using EIF4G2 Rabbit Polyclonal Antibody orb213886 with dilution at 1:100.

Quick Database Links

Gene Symbol

EIF4G2

UniProt

Q62448, P78344

NCBI Gene ID

13690, 1982

UniProt Details

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No UniProt data available

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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Immunofluorescence

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