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GFAP Antibody
SKU: orb43801
Description
Research Area
Epigenetics, Neuroscience
Images & Validation
−Item 1 of 2
| Tested Applications | ICC, IHC-Fr, IHC-P, WB |
|---|---|
| Reactivity | Human, Porcine |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Clonality | Monoclonal |
| Isotype | Mouse IgM |
| Clone No. | GF-02 |
| Immunogen | Pellet of porcine brain cold-stable proteins after depolymerization of microtubules. |
| Target | GFAP |
| Purification | Purified by sequential steps of physicochemical fractionation (differential precipitation and solid-phase chromatography methods). |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Tris buffered saline (TBS), pH 8.0, 15 mM sodium azide |
| Concentration | 1 mg/ml |
| Disclaimer | For research use only |
Alternative Names
−Anti-GFAP antibody
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Western blotting analysis of human GFAP using mouse monoclonal antibody GF-02 on lysates of Neuro 2a and HEK 293T cells under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse anti-GFAP monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. A specific band was detected for GFAP at approximately 54 kDa.
Western blotting analysis (reducing conditions) of GFAP in porcine brain lysate. Lane 1: immunostaining with anti-GFAP (GF-02); Lane 2: immunostaining with Isotype mouse IgM control (PFR-03; cat. no. orb43568).
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IHC-Fr
Immunohistochemistry Frozen
ICC
Immunocytochemistry
GFAP Antibody (orb43801)
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