Histone H3 (DiMethyl K27) Antibody

SKU: orb2649412

Description

Mouse monoclonal antibody to Histone H3 (DiMethyl K27)

Images & Validation

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Item 1 of 3

Tested Applications	IHC, IP, WB
Dilution range	WB: WB (1/1000 - 1/3000), IH (1/200 - 1/500), IP (1/50 - 1/200), IP: WB (1/1000 - 1/3000), IH (1/200 - 1/500), IP (1/50 - 1/200)
Reactivity	Human, Mouse, Rat

Key Properties

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Host	Mouse
Clonality	Monoclonal
Clone No.	HIST1H3A; HIST1H3B; HIST1H3C; HIST1H3D; HIST1H3E; HIST1H3F; HIST1H3G; HIST1H3H; HIST1H3I; HIST1H3J;
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Disclaimer	For research use only

Alternative Names

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HIST1H3A; H3FA; HIST1H3B; H3FL; HIST1H3C; H3FC; HIST1H3D; H3FB; HIST1H3E; H3FD; HIST1H3F; H3FI; HIST1H3G; H3FH; HIST1H3H; H3FK; HIST1H3I; H3FF; HIST1H3J; H3FJ; Histone H3.1; Histone H3/a; Histone H3/b; Histone H3/c; Histone H3/d; Histone H3/f; Histone H3/h; Histone H3/i; Histone H3/j; Histone H3/k; Histone H3/l; HIST2H3A; HIST2H3C; H3F2; H3FM; HIST2H3D; Histone H3.2; Histone H3/m; Histone H3/o; H3F3A; H3.3A; H3F3; PP781; H3F3B; H3.3B; Histone H3.3

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Western blot analysis of Histone H3 (DiMethyl K27) expression in Hela (A) whole cell lysates.

Immunoprecipitation of Histone H3 (DiMethyl K27) from 0.5mg Hela whole cell extract lysate, using Anti-Histone H3 (DiMethyl K27) Antibody.

Immunohistochemical analysis of Histone H3 (DiMethyl K27) staining in human skin formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Quick Database Links

UniProt

P68431, Q71DI3, P84243

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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Immunoprecipitation

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