DiMethyl-Histone H3-K27 polyclonal antibody

SKU: orb647951

Description

Rabbit polyclonal antibody to Histone H3 (Di-Methyl K27)

Images & Validation

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Tested Applications	ChIP, IF, IP, WB
Dilution range	WB1:500 - 1:2000, IHC-P: -P1:50 - 1:200, IF1:50 - 1:200IP1:50 - 1:200, ChIP1:20 - 1:100, ChIPseq1:20 - 1:100
Reactivity	Human, Mouse, Other, Rat

Key Properties

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Antibody Type	Primary Antibody
Host	Rabbit
Clonality	Polyclonal
Immunogen	A synthetic dimethylated peptide around K27 of human histone H3(NP_003520.1).
Molecular Weight	17KDa
Purification	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen and the purity is > 95% (by SDS-PAGE).
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH 7.2
Buffer/Preservatives	Rabbit IgG, 1mg/ml in PBS with 0.02% sodium azide, 50% glycerol, pH 7.2
Concentration	1mg/ml
Disclaimer	For research use only

Alternative Names

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H3.4;H3/g;H3FT;H3t;HIST3H3;Histone H3;HIST1H3A

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Quick Database Links

UniProt

Q16695

UniProt Details

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No UniProt data available

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Protocol Information

Western Blot (IB, immunoblot)

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Immunofluorescence

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Immunoprecipitation

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ChIP

Chromatin Immunoprecipitation

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