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HLA-F Antibody (PE)
SKU: orb545717
Description
Research Area
Epigenetics, Immunology
Images & Validation
−Item 1 of 4
| Tested Applications | FC |
|---|---|
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Clonality | Monoclonal |
| Isotype | Mouse IgG1 |
| Clone No. | 3D11 |
| Immunogen | Inclusion body-derived HLA-F heavy chain |
| Target | HLA-F |
| Purification | Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography. |
| Conjugation | PE |
Storage & Handling
−| Storage | Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze. |
|---|---|
| Buffer/Preservatives | Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Concentration | 0.1 mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−CDA12
Quality Guarantee
Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].
Flow cytometry multicolor intracellular staining pattern of human lymphocytes stained using anti-human CD20 (2H7) PE antibody (10 μl reagent / 100 μl of peripheral whole blood) and anti-HLA-F (3D11) PE antibody (concentration in sample 5 μg/ml, red) or mouse IgG1 isotype control (MOPC-21) PE antibody (concentration in sample 5 μg/ml, same as anti-HLA-F PE concentration, black).
Flow cytometry multicolor surface staining pattern of human lymphocytes stained using anti-human CD20 (2H7) PE antibody (10 μl reagent / 100 μl of peripheral whole blood) and anti-HLA-F (3D11) PE antibody (concentration in sample 5 μg/ml, red) or mouse IgG1 isotype control (MOPC-21) PE antibody (concentration in sample 5 μg/ml, same as anti-HLA-F PE concentration, black).
Separation of lymphocytes stained using anti-HLA-F (3D11) PE antibody (concentration in sample 5 μg/ml, red-filled) from lymphocytes stained using mouse IgG1 isotype control (MOPC-21) PE antibody (concentration in sample 5 μg/ml, same as anti-HLA-F PE concentration, black-dashed) in flow cytometry analysis (intracellular staining) of human stimulated (PMA + Ionomycin) whole blood cells.
Separation of lymphocytes stained using anti-HLA-F (3D11) PE antibody (concentration in sample 5 μg/ml, red-filled) from lymphocytes stained using mouse IgG1 isotype control (MOPC-21) PE antibody (concentration in sample 5 μg/ml, same as anti-HLA-F PE concentration, black-dashed) in flow cytometry analysis (surface staining) of human stimulated (PMA + Ionomycin) whole blood cells.
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HLA-F Antibody (PE) (orb545717)
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