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Description
Research Area
Protein Biochemistry
Images & Validation
−Item 1 of 1
| Tested Applications | ELISA, IHC-P, RIA, WB |
|---|---|
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Clonality | Monoclonal |
| Isotype | Mouse IgG1 |
| Clone No. | AL-01 |
| Immunogen | Fraction of proteins containing albumin after ammonnium sulphate precipitation and DEAE-chromatography of human serum. |
| Target | Albumin |
| Purification | Purified antibody is conjugated with biotin LC-NHS ester under optimum conditions and unconjugated antibody and free biotin are removed by size-exclusion chromatography. |
| Conjugation | Biotin |
Storage & Handling
−| Storage | Store at 2-8°C. Do not freeze. |
|---|---|
| Buffer/Preservatives | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Concentration | 1 mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−HSA, SA
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Western blotting analysis of human serum albumin using mouse monoclonal antibody AL-01 on human plasma and lysate of HeLa cell line (albumin non-expressing cell line; negative control) under non-reducing and reducing conditions. Nitrocellulose membrane was probed with 5 µg/ml of biotinylated mouse anti-human albumin monoclonal antibody followed by IRDye800-conjugated streptavidin. Albumin was detected at approximately 50-67 kDa.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
RIA
Radioimmunoassay
Albumin Antibody (Biotin) (orb43650)
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