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BCL2 Antibody (PE)

SKU: orb44690

Description

Mouse monoclonal antibody against BCL2 conjugated to PE

Images & Validation

Tested ApplicationsFC
ReactivityHuman
Application Notes
Flow cytometry: The reagent is designed for analysis of human blood cells using 20 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (2 ml) is sufficient for 100 tests. Intracellular staining.

Key Properties

Antibody TypePrimary Antibody
ClonalityMonoclonal
IsotypeMouse IgG1
Clone No.Bcl-2/100
ImmunogenSynthetic peptide corresponding to the amino acids 41-54 of human Bcl2
TargetBCL2
PurificationPurified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
ConjugationPE

Storage & Handling

StorageStore at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Buffer/PreservativesStabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
DisclaimerFor research use only

Alternative Names

Bcl-2, PPP1R50

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BCL2 Antibody (PE)

Flow cytometry multicolor staining of human lymphocytes stained using anti-human BCL2 (Bcl-2/100) PE antibody (intracellular staining, 10 µl reagent / 100 µl of peripheral whole blood from DLBCL patient) and anti-human CD19 (LT19) FITC antibody (surface staining, 20 µl reagent / 100 µl of peripheral whole blood from DLBCL patient).

BCL2 Antibody (PE)

Separation of human BCL-2 positive CD19 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (intracellular staining) of human peripheral whole blood from DLBCL patient stained using anti-human BCL2 (Bcl-2/100) PE antibody (10 µl reagent / 100 µl of peripheral whole blood).

BCL2 Antibody (PE)

Flow cytometry intracellular staining pattern of human peripheral whole blood from diffuse large B-cell lymphoma (DLBCL) patient. Sample was stained using anti-human BCL2 (Bcl-2/100) PE antibody (10 µl reagent / 100 µl of peripheral whole blood).

BCL2 Antibody (PE)

Flow cytometry intracellular staining pattern of human peripheral whole blood stained using anti-human BCL2 (Bcl-2/100) PE antibody (concentration in sample 1.67 µg/ml).

BCL2 Antibody (PE)

Separation of human BCL2 positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (intracellular staining) of human peripheral whole blood stained using anti-human BCL2 (Bcl-2/100) PE antibody (concentration in sample 1.67 µg/ml).

UniProt Details

No UniProt data available

NCBI Gene Details

No NCBI Gene data available

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Protocol Information

FC
Flow Cytometry
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BCL2 Antibody (PE) (orb44690)

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100 T
¥ 6,500.00
DispatchUsually dispatched within 5-10 working days.
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