CD253 Antibody (PE)

SKU: orb154497

Description

Mouse monoclonal antibody conjugated to PE which recognizes CD253 that is also known as TNFSF10 is a cytokine that is a member of the tumor necrosis factor (TNF) ligand family. It highly expressed in spleen, lung and prostate. CD253 is commonly used as cell markers in immunophynotyping and also induces apoptosis

Research Area

Apoptotic, Cancer, Epigenetics, Neuroscience, Signaling Pathways

Images & Validation

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Item 1 of 1

Tested Applications	FC
Reactivity	Human
Application Notes	Flow cytometry: Recommended dilution: 1-5 μg/ml.

Key Properties

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Antibody Type	Primary Antibody
Clonality	Monoclonal
Isotype	Mouse IgG1
Clone No.	2E5
Immunogen	Recombinant soluble fragment (aa 95-281) of human TRAIL.
Target	CD253
Purification	Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Conjugation	PE

Storage & Handling

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Storage	Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Buffer/Preservatives	Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Concentration	0.1 mg/ml
Disclaimer	For research use only

Alternative Names

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TRAIL, Apo-2 ligand, Apo-2L, TNFSF10, APO2L, TNLG6A

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Anti-Hu CD253 PE antibody (clone 2E5) specificity verification by flow cytometry Colorectal cancer cell line HCT-116 with the eliminated expression of pro-apoptotic proteins BAK and BAX (prepared using CRISPR Cas9 gene editing approach) was used as host for CD253 (TRAIL) transfection. BAK/BAX double knockout cells are resistant to TRAIL-induced apoptosis. Anti-Hu CD253 PE (concentration in sample 5 μg/ml, red-filled histogram) binds specifically to surface CD253/TRAIL in transfected cells (upper panel), but not to the parent HCT-166 BAK/BAK double KO (lower panel). Level of non-specific binding was assessed using Mouse IgG1 isotype control PE (MOPC-21) under same conditions (concentration in sample 5 μg/ml, black-dashed histogram).

Quick Database Links

Gene Symbol

CD253

UniProt

P50591

NCBI Gene ID

8743

UniProt Details

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No UniProt data available

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Flow Cytometry

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