CD314 Antibody (FITC)

SKU: orb154477

Description

Mouse monoclonal antibody conjugated to FITC which recognizes CD314 that is also known as BAT221, NKG2D a cell surface receptor of the type II transmembrane receptor NKG2 family. CD314 is expressed on NK cells and some T cell subsets, where it functions as an activating receptor to trigger cytolytic activity and cytokine secretion.

Research Area

Epigenetics, Immunology

Images & Validation

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Item 1 of 3

Tested Applications	FC
Reactivity	Human
Application Notes	Flow cytometry: The reagent is designed for analysis of human blood cells using 4 μl reagent / 100 μl of whole blood or 106 cells in a suspension. The content of a vial (0.4 ml) is sufficient for 100 tests.

Key Properties

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Antibody Type	Primary Antibody
Clonality	Monoclonal
Isotype	Mouse IgG1 kappa
Clone No.	1D11
Immunogen	NKL cell line
Target	CD314
Purification	Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Conjugation	FITC

Storage & Handling

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Storage	Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Buffer/Preservatives	Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Disclaimer	For research use only

Alternative Names

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NKG2D, KLRK1, KLR

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Flow cytometry multicolor surface staining of human lymphocytes stained using anti-human CD314 (1D11) FITC antibody (4 µl reagent / 100 µl of peripheral whole blood) and anti-human CD56 (LT56) APC antibody (10 µl reagent / 100 µl of peripheral whole blood).

Separation of human CD314 positive CD56 positive NK cells (red-filled) from CD314 negative CD56 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD314 (1D11) FITC antibody (4 µl reagent / 100 µl of peripheral whole blood).

Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD314 (1D11) FITC antibody (4 µl reagent / 100 µl of peripheral whole blood).

Quick Database Links

Gene Symbol

CD314

UniProt

P26718

NCBI Gene ID

22914

UniProt Details

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No UniProt data available

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Flow Cytometry

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