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CD45RA Antibody
SKU: orb44329
Description
Images & Validation
−Item 1 of 5
| Tested Applications | FC, IHC-P, IP, WB |
|---|---|
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Clonality | Monoclonal |
| Isotype | Mouse IgG2b |
| Clone No. | MEM-56 |
| Immunogen | Human thymocytes and T lymphocytes. |
| Target | CD45RA |
| Purification | Purified by protein-A affinity chromatography. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Concentration | 1 mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−PTPRCR, T200RA
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Mass cytometry (surface staining) of human peripheral blood with anti-CD45RA (MEM-56) 163Dy. Gated on singlets.
Western blotting analysis of whole cell lysate of HUT-78 human cutaneous T cell lymphoma cell line. Lane 1: immunostaining with anti-human CD45 control antibody; Lane 2: immunostaining with anti-human CD45RA (MEM-56); sample of cell lysate prepared under non-reducing conditions; Lane 3: immunostaining with anti-human CD45RA (MEM-56); sample of cell lysate prepared under reducing conditions.
Separation of human CD45RA positive lymphocytes (red-filled) from neutrophil granulocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral blood stained using anti-human CD45RA (MEM-56) purified antibody (concentration in sample 0.3 µg/ml, GAM APC).
Flow cytometry surface staining pattern of human peripheral blood stained using anti-human CD45RA (MEM-56) purified antibody (concentration in sample 0.3 µg/ml, GAM APC).
Immunohistochemistry staining of human tonsil (paraffin-embedded sections) with anti-CD45RA (MEM-56), 5 µg/ml.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
FC
Flow Cytometry
IP
Immunoprecipitation
CD45RA Antibody (orb44329)
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