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CD54 Antibody
SKU: orb43579
Description
Research Area
Epigenetics, Infectious Diseases
Images & Validation
−Item 1 of 3
| Tested Applications | FC, ICC, IHC-Fr, IHC-P, WB |
|---|---|
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Clonality | Monoclonal |
| Isotype | Mouse IgG2b |
| Clone No. | 1H4 |
| Immunogen | Raji cells and spleen cells fused with NS1 cells |
| Target | CD54 |
| Purification | Purified by protein-A affinity chromatography. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Phosphate buffered saline (PBS), pH 7.4 |
| Concentration | 1 mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−ICAM-1, BB2, P3.58
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Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD54 (1H4) purified antibody (concentration in sample 3 μg/ml, GAM FITC).
Western blotting analysis of human CD54 using mouse monoclonal antibody 1H4 on lysates of TF-1 and Raji cells, as well as of JURKAT cells (negative control) under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse anti-CD54 monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. A specific band was detected for CD54 at approximately 88 kDa.
Separation of human monocytes (red-filled) from human lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of peripheral whole blood stained using anti-human CD54 (1H4) purified antibody (concentration in sample 3 μg/ml, GAM FITC).
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC-P
Immunohistochemistry Paraffin
IHC-Fr
Immunohistochemistry Frozen
FC
Flow Cytometry
ICC
Immunocytochemistry
CD54 Antibody (orb43579)
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