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Description
Research Area
Immunology & Inflammation; Cell Biology
Images & Validation
−Item 1 of 3
| Tested Applications | FC, IHC-P |
|---|---|
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Clonality | Monoclonal |
| Isotype | Mouse IgG1 |
| Clone No. | LT95 |
| Immunogen | HUT-78 human T cell lymphoma cell line |
| Target | CD95 |
| Purification | Purified by protein-A affinity chromatography. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Concentration | 1 mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−FAS1, APT1, APO-1, FASTM, ALPS1A, TNFRSF6
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Flow cytometry analysis of Fas (CD95) expression on the surface of various hematopoietic cell lines with purified anti-human CD95 (LT95) (detection by Goat anti-mouse IgG1 PE). Histograms: Red - Isotype mouse IgG1 control staining; Black - standard anti-CD95 monoclonal antibody (DX2); Green - anti-CD95 (LT95). A - JURKAT human peripheral blood T cell leukemia cell line; B - RAMOS human Burkitt lymphoma cell line; C - CEM human leukemia cell line; D - MOLT-4 human acute lymphoblastic T cell leukemia cell line.
Separation of human CD95 positive lymphocytes (red-filled) from CD95 negative lymphocytes (black-dashed) in flow cytometry analysis (surface staining) of human peripheral whole blood stained using anti-human CD95 (LT95) purified antibody (concentration in sample 2 µg/ml) GAM APC.
Flow cytometry surface staining pattern of human peripheral whole blood stained using anti-human CD95 (LT95) purified antibody (concentration in sample 2 µg/ml) GAM APC.
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Protocol Information
IHC-P
Immunohistochemistry Paraffin
FC
Flow Cytometry
CD95 Antibody (orb43957)
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