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EGFR (pY1173) Antibody
SKU: orb43776
Description
Images & Validation
−Item 1 of 4
| Tested Applications | FC, IP, WB |
|---|---|
| Reactivity | Human |
| Application Notes |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Clonality | Monoclonal |
| Isotype | Mouse IgG1 |
| Clone No. | EM-13 |
| Immunogen | Synthetic phospho-peptide covering sequence around tyrosine 1173 of human EGFR. |
| Target | EGFR (pY1173) |
| Purification | Purified by protein-A affinity chromatography. |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Concentration | 1 mg/ml |
| Disclaimer | For research use only |
Alternative Names
−EGFR, EGF-R, Her1, ERBB1, PIG61, NISBD2
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Immunoprecipitation of EGFR from EGF-treated A431 cells by monoclonal antibodies EM-12 (A), EM-13 (B), a commercial anti-EGFR polyclonal antibody (C) and anti-EGFR monoclonal mAb108 (D). The precipitates were immunoblotted with EM-12 or EM-13 antibody, and goat anti-mouse-HRP.
Immunoprecipitation of EGFR from EGF-treated A431 cells by phosphospecific monoclonal antibodies EM-12 (A), EM-13 (B), a commercial anti-EGFR polyclonal antibody (C) and anti-EGFR monoclonal mAb108 (D). The precipitates were immunoblotted with a commercial anti-EGFR polyclonal and goat anti-rabbit-HRP.
Western blotting analysis of EGFR (phospho-Tyr1173) by mouse monoclonal antibody EM-13 in EGF-treated A431 (A), CALU-3 (B), MCF-7 (C), Jurkat (D) and Ramos (E) cell lines (reduced conditions).
Separation of EGF stimulated A431 cell suspension stained using anti-human EGFR (pY1173) (EM-13) purified antibody (concentration in sample 3 µg/ml, GAM PE, red-filled) from EGF stimulated A431 cell suspension unstained by primary antibody (GAM PE, black-dashed) in flow cytometry analysis (intracellular staining).
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Protocol Information
WB
Western Blot (IB, immunoblot)
FC
Flow Cytometry
IP
Immunoprecipitation
EGFR (pY1173) Antibody (orb43776)
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