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IL-2 Antibody
SKU: orb1562578
Description
Images & Validation
−Item 1 of 3
| Tested Applications | ELISA, FC, ICC, IP, WB |
|---|---|
| Reactivity | Human |
| Application Notes |
Key Properties
−| Clonality | Monoclonal |
|---|---|
| Isotype | Mouse IgG2b kappa |
| Clone No. | 35C3 |
| Immunogen | recombinant human IL-2 |
| Target | IL-2 |
| Purification | Purified by protein-A affinity chromatography. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide |
| Concentration | 1 mg/ml |
| Disclaimer | For research use only |
Alternative Names
−Interleukin 2
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Flow cytometry multicolor intracellular staining of human PMA + ionomycin stimulated and Brefeldin A treated peripheral whole blood showing lymphocytes stained using anti-human CD4 (MEM-241) PE-Cy™7 antibody (4 μl reagent / 100 μl of peripheral whole blood) and anti-human IL-2 (35C3) purified antibody (concentration in sample 0.5 μg/ml, GAM APC).
Separation of human CD4 positive IL-2 positive lymphocytes (red-filled) from CD4 negative IL-2 negative lymphocytes (black-dashed) in flow cytometry analysis (intracellular staining) of human PMA + ionomycin stimulated and Brefeldin A treated peripheral whole blood stained using anti-human IL-2 (35C3) purified antibody (concentration in sample 0.5 μg/ml, GAM APC).
Flow cytometry intracellular staining pattern of human PMA + ionomycin stimulated and Brefeldin A treated peripheral whole blood stained using anti-human IL-2 (35C3) purified antibody (concentration in sample 0.5 μg/ml, GAM APC).
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Protocol Information
WB
Western Blot (IB, immunoblot)
FC
Flow Cytometry
ICC
Immunocytochemistry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
IP
Immunoprecipitation
IL-2 Antibody (orb1562578)
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