IFN gamma Antibody

SKU: orb154408

Description

Mouse monoclonal antibody against IFN gamma

Research Area

Epigenetics

Images & Validation

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Item 1 of 2

Tested Applications	ELISA, FC, ICC, IHC-P, IP, RIA, WB
Reactivity	Human, Primate
Application Notes	ELISA: This antibody is being used as detection antibody in combination with capture antibody NIB42. Flow cytometry: Recommended dilution: 1-4 µg/ml. Intracellular staining.

Key Properties

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Antibody Type	Primary Antibody
Clonality	Monoclonal
Isotype	Mouse IgG1 kappa
Clone No.	4S.B3
Immunogen	Interferon gamma derived from human leukocytes
Target	IFN gamma
Purification	Purified by protein-A affinity chromatography.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Concentration	1 mg/ml
Disclaimer	For research use only

Alternative Names

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Interferon gamma, IFN-gamma

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Separation of human IFN gamma positive lymphocytes (red-filled) from IFN gamma negative lymphocytes (black-dashed) in flow cytometry analysis (intracellular staining) of human PHA stimulated and Brefeldin A treated peripheral blood mononuclear cells stained using anti-IFN gamma (4S.B3) purified antibody (concentration in sample 4 μg/ml, GAM APC).

Flow cytometry intracellular staining pattern of human PHA stimulated and Brefeldin A treated peripheral blood mononuclear cells stained using anti-IFN gamma (4S.B3) purified antibody (concentration in sample 4 μg/ml, GAM APC).