Anti-IFN-gamma receptor 1 [GR20]

SKU: orb1089965

Description

Rat monoclonal antibody to IFN-gamma receptor 1

Research Area

Cancer Biology, Immunology & Inflammation

Images & Validation

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Item 1 of 2

Tested Applications	Blocking, FC
Reactivity	Mouse

Key Properties

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Host	Rat
Clonality	Monoclonal
Isotype	IgG2a
Clone No.	GR20
Immunogen	This antibody was raised by immunising rats with interferon gamma receptor.
Target	IFN-gamma receptor 1
Purity	Purified
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	PBS with 0.02% Proclin 300.
Concentration	1 mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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CD119; Interferon gamma receptor 1; IFN gamma receptor 1; IFN-gamma-R1; Interferon gamma receptor alpha-chain; IFN-gamma-R-alpha

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Flow cytometry using the Anti-IFN-gamma receptor 1 antibody GR20. Paraformaldehyde fixed mouse splenocytes permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (orb256458; isotype control, black line) or the rabbit IgG version of GR20 (orb1089966, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of mouse splenocytes with anti-IFN-gamma receptor 1 GR20. Immunofluorescence analysis of paraformaldehyde fixed mouse splenocytes on Shi-fix™ cover-slips stained with the chimeric rabbit IgG version of GR20 (orb1089966) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing membrane and cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb1089966, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (orb256458) followed by staining with Alexa Fluor® 488 secondary antibody.

Quick Database Links

Gene Symbol

IFN-gamma receptor 1

UniProt

P15261

UniProt Details

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No UniProt data available

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Protocol Information

Flow Cytometry

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