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IL-5 Antibody
SKU: orb1806163
Description
Images & Validation
−Item 1 of 5
| Tested Applications | ELISA, FA, FACS, In vivo |
|---|---|
| Reactivity | Human |
Key Properties
−| Clonality | Monoclonal |
|---|---|
| Target | IL-5 |
| Source/Expression System | CHO |
| Molecular Weight | 146.24 kDa |
| Purity | >95% |
| Purification | Protein A |
| Endotoxin | <0.001 EU/ug |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | 100 mM Proline/Acetic acid 20mM Arginine pH 5.0 |
| Concentration | Batch dependent |
| Disclaimer | For research use only |
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Anti-IL-5 Reference Antibody (mepolizumab) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.
The purity of Anti-IL-5 Reference Antibody (mepolizumab)is more than 98.47% ;determined by SEC-HPLC.
Immobilized human IL 5 His at 2 μg/mL can bind Anti-IL-5 Reference Antibody (mepolizumab);EC50=0.1188μg/mL.
Anti-IL-5 Reference Antibody (mepolizumab) FACS Blocking was evaluated using Human IL-5Rβ-FL/HIL-5Rα CHOS. The IC50 was approximately 2.844 ug/mL.
Mepolizumab induced proliferation inhibition activity was evaluated using Human IL-5Rα TF-1 . The EC50 was approximately 0.3282 ug/mL.
Quick Database Links
Gene Symbol
IL-5
UniProt
UniProt Details
− No UniProt data available
Documents Download
Datasheet
Product Information
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Protocol Information
FACS
Fluorescence-Activated Cell Sorting (FC, Flow cytometry)
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
IL-5 Antibody (orb1806163)
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