Anti-IL2R beta [5H4]

SKU: orb758865

Description

Rat monoclonal antibody to IL2R beta

Research Area

Immunology & Inflammation

Images & Validation

−

Item 1 of 2

Tested Applications	FC
Reactivity	Mouse

Key Properties

−

Host	Rat
Clonality	Monoclonal
Isotype	IgG2a
Clone No.	5H4
Immunogen	This antibody was raised by immunising Lewis rats with transfected rat myeloma cell line YB2/0, expressing truncated murine IL-2R beta.
Target	IL2R beta
Purity	Purified
Conjugation	Unconjugated

Storage & Handling

−

Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	PBS with 0.02% Proclin 300.
Concentration	1 mg/ml
Disclaimer	For research use only

Alternative Names

−

CD122; IL-2Rβ; IL-2 receptor subunit beta; Interleukin-2 receptor subunit beta; High affinity IL-2 receptor subunit beta; p70-75

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Flow cytometry using the Anti-IL2R beta antibody 5H4. Paraformaldehyde fixed mouse splenocytes permeabilized with 0.5% Triton were stained with anti-unknown specificity antibody (orb256458; isotype control, black line) or the rabbit IgG version of 5H4 (orb758864, blue line) at a dilution of 1:100 for 1h at RT. After washing, the bound antibody was detected using a goat anti-rabbit IgG AlexaFluor® 488 antibody at a dilution of 1:1000 and cells analyzed using a FACSCanto flow-cytometer.

Immunofluorescence staining of fixed RAW264.7 cells with anti-IL2R beta antibody 5H4. Immunofluorescence analysis of paraformaldehyde fixed RAW264.7 cells on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of 5H4 (orb758864) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb758864, DAPI, merged channels and an isotype control. The isotype control was an unknown specificity antibody (orb256449) followed by staining with Alexa Fluor® 488 secondary antibody.

Quick Database Links

Gene Symbol

IL2R beta

UniProt

P16297

UniProt Details

−

No UniProt data available

Documents Download

Datasheet

Product Information

Download

Request a Document

Protocol Information

Flow Cytometry

View Protocol

Find More Protocols