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Description
Research Area
Cancer Biology
Images & Validation
−Item 1 of 1
| Tested Applications | ELISA, IHC |
|---|---|
| Reactivity | Human |
Key Properties
−| Host | Mouse |
|---|---|
| Clonality | Monoclonal |
| Isotype | Mouse IgG |
| Clone No. | BR96 |
| Immunogen | H3396 cultured line established from a metastasis of a human breast adenocarcinoma. |
| Target | LeY |
| Purity | Purified |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Buffer/Preservatives | PBS with 0.02% Proclin 300. |
| Concentration | 1 mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Lewis Y
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![Anti-LeY [BR96]](/images/pub/media/catalog/product/NewWebsite/35/orb613860_1.png)
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![Anti-LeY [BR96]](/images/pub/media/catalog/product/NewWebsite/35/orb613861_1.png)
Immunofluorescence staining of fixed Caco-2 cells with anti-LeY tetrasaccharide antibody BR96. Immunofluorescence analysis of paraformaldehyde fixed Caco-2 cells on Shi-fix™ coverslips, permeabilized with 0.15% Triton and stained with the chimeric mouse IgG1 version of BR96 (orb613860) at 10 µg/ml for 1h followed by Alexa Fluor® 488 secondary antibody (2 µg/ml), showing membrane staining. The nuclear stain is DAPI (blue). Panels show from left-right, top-bottom orb613860, DAPI, merged channels and an isotype control. The isotype control was stained with an unknown specificity antibody (orb256449) followed by Alexa Fluor® 488 secondary antibody.
Quick Database Links
Gene Symbol
LeY
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Datasheet
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Protocol Information
IHC
Immunohistochemistry
ELISA
Enzyme-linked Immunosorbent Assay (EIA)
Anti-LeY [BR96] (orb613861)
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