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MAdCAM1 Rabbit Polyclonal Antibody
SKU: orb654439
Description
Research Area
Cardiovascular Research, Cell Biology, Immunology & Inflammation, Neuroscience, Signal Transduction
Images & Validation
−Item 1 of 2
| Tested Applications | IHC |
|---|---|
| Dilution Range | Immunohistochemistry (Paraffin-embedded Section), 0.5-1μg/ml, Human |
| Reactivity | Human |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Immunogen | A synthetic peptide corresponding to a sequence in the middle region of human MAdCAM1, which shares 56.5% amino acid (aa) sequence identity with mouse MAdCAM1. |
| Target | Mucosal addressin cell adhesion molecule 1 |
| Molecular Weight | 43 kDa |
| Purification | Immunogen affinity purified. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | Each vial contains 4mg Trehalose, 0.9mg NaCl, 0.2mg Na2HPO4, 0.01mg NaN3. |
| Concentration | 500 µg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−hMAdCAM 1; MACAM1; MAdCAM1
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IHC analysis of MAdCAM14 using anti-MAdCAM1 antibody. MAdCAM1 was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 µg/ml rabbit anti-MAdCAM1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
IHC analysis of MAdCAM14 using anti-MAdCAM1 antibody. MAdCAM1 was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1 µg/ml rabbit anti-MAdCAM1 Antibody overnight at 4°C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37°C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.
Quick Database Links
Gene Symbol
Mucosal addressin cell adhesion molecule 1
UniProt
UniProt Details
− No UniProt data available
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MAdCAM1 Rabbit Polyclonal Antibody (orb654439)
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