Anti-Myelin basic protein [F28C4]

SKU: orb2645602

Description

Research Area

Neuroscience

Images & Validation

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Item 1 of 2

Tested Applications	ELISA
Reactivity	Human

Key Properties

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Host	Mouse
Clonality	Monoclonal
Isotype	IgG2a
Clone No.	F28C4
Immunogen	The original antibody was generated by immunizing PL/J mice with MBP acetyl residues 1-9.
Target	Myelin basic protein
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	PBS with 0.02% Proclin 300.
Concentration	1 mg/ml
Disclaimer	For research use only

Alternative Names

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MBP; Myelin A1 protein; Myelin membrane encephalitogenic protein

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Immunofluorescence staining of Kelly cells with anti-Myelin basic protein antibody F28C4. Immunofluorescence analysis of paraformaldehyde-fixed Kelly cells on Shi-fix™ coverslips stained with the chimeric rabbit IgG version of F28C4 (orb2645601) (1:100 dilution) for 1h followed by Alexa Fluor® 488 secondary antibody (1:1000 dilution), showing cytoplasmic staining. The nuclear stain is DAPI (blue). Panels show, from left-right, top-bottom, orb2645601, DAPI, merged channels, and an isotype control. The isotype control was an unknown specificity antibody (orb256458) followed by staining with Alexa Fluor® 488 secondary antibody.

Western blot using anti-Myelin basic protein antibody F28C4. Human brain cerebellum (A) (0.0001 µg/ml) and hippocampus (B) (5 µg/ml) tissue lysates (5 µg protein in RIPA buffer) and cerebral cortex (C) (0.001 µg/ml) tissue lysate (35 µg protein in RIPA buffer) were resolved via SDS-PAGE, and the subsequent blots were probed with the chimeric rabbit version of F28C4 (orb2645601) at the mentioned respective concentrations before detection using an anti-rabbit secondary antibody. A primary incubation of 1 hour was used, and proteins were detected by chemiluminescence.

Quick Database Links

Gene Symbol

Myelin basic protein

UniProt

P02686

UniProt Details

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No UniProt data available

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Protocol Information

ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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