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p38 (Phospho-T180) Antibody
SKU: orb216074
Featured
Description
Research Area
Apoptotic, Cancer, Epigenetics, Neuroscience, Signaling Pathways
Images & Validation
−Item 1 of 4
| Tested Applications | IF, WB |
|---|---|
| Dilution range | WB: 1:500-1:1000, IF/ICC: 1:100-1:500, IP: 1:10-1:100 |
| Reactivity | Canine, Human, Mouse, Rat, Zebrafish |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Immunogen | KLH-conjugated synthetic phosphopeptide corresponding to residues surrounding T180 of human p38 protein. The exact sequence is proprietary. |
| Purification | The antibody was purified by immunogen affinity chromatography. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide. |
| Disclaimer | For research use only |
Alternative Names
−CSBP; CSBP1; CSBP2; CSPB1; MXI2; SAPK2A; Mitogen-activated protein kinase 14; MAP kinase 14; MAPK 14; Cytokine suppressive anti-inflammatory drug-binding protein; CSAID-binding protein; CSBP; MAP kinase MXI2; MAX-interacting protein 2; Mitogen-activated protein kinase p38 alpha; MAP kinase p38 alpha; Stress-activated protein kinase 2a; SAPK2a
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Western blot analysis of p38 (Phospho-T180) expression in zebrafish (A) whole cell lysates. (Predicted band size: 41 kD; Observed band size: 43 kD)
Immunofluorescent analysis of p38 (Phospho-T180) staining in HepG2 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.
Western blot analysis of p38 (Phospho-T180) expression in mouse liver (A), rat liver (B) whole cell lysates.
Direct ELISA antibody dose-response curve using Anti-p38 (Phospho-T180) Antibody. Antigen (Phosphopeptide and non-phosphopeptide) concentration is 5 ug/ml. Goat Anti-Rabbit IgG (H&L) - HRP was used as the secondary antibody, and signal was developed by TMB substrate.
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Protocol Information
WB
Western Blot (IB, immunoblot)
IF
Immunofluorescence
p38 (Phospho-T180) Antibody (orb216074)
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