p53 Antibody (FITC)

SKU: orb43683

Description

Mouse monoclonal antibody against p53 conjugated to FITC.

Research Area

Apoptotic, Cancer, Epigenetics, Infectious Diseases, Neuroscience, Signaling Pathways

Images & Validation

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Item 1 of 1

Tested Applications	FC
Reactivity	Human, Primate
Application Notes	Flow cytometry: Recommended dilution: 3 μg/ml. Intracellular staining.

Key Properties

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Antibody Type	Primary Antibody
Clonality	Monoclonal
Isotype	Mouse IgG2a
Clone No.	BP53-12
Immunogen	Bacterially expressed full-length wild-type p53
Target	p53
Purification	Purified antibody is conjugated with fluorescein isothiocyanate (FITC) under optimum conditions and unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Conjugation	FITC

Storage & Handling

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Storage	Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Buffer/Preservatives	Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Concentration	1 mg/ml
Disclaimer	For research use only

Alternative Names

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BCC7, TRP53, TP53, LFS1

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Separation of Ramos cells stained using anti-p53 (BP53.12) FITC antibody (concentration in sample 9 µg/ml, red) from unstained Ramos cells (black) in flow cytometry analysis (intracellular staining).

Quick Database Links

Gene Symbol

p53

UniProt

P04637

NCBI Gene ID

7157

UniProt Details

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No UniProt data available

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Flow Cytometry

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