Phosphotyrosine Antibody

SKU: orb44476

This product can be custom made

Description

Mouse Monoclonal to Phosphotyrosine.

Images & Validation

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Item 1 of 1

Tested Applications	FC, ICC, WB
Reactivity	All
Application Notes	Western blotting: Recommended dilution: 1-2 μg/ml. Flow cytometry: Recommended dilution: 2-5 μg/ml. Intracellular staining.

Key Properties

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Antibody Type	Primary Antibody
Clonality	Monoclonal
Isotype	Mouse IgG1
Clone No.	P-Tyr-01
Immunogen	Phosphotyrosine conjugated to bovine serum albumin.
Target	Phosphotyrosine
Purification	Purified by protein-A affinity chromatography.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	Phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Concentration	1 mg/ml
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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Anti-Phosphotyrosine purified antibody (clone P-Tyr-01) Specificity Verification by Flow Cytometry Anti-Phosphotyrosine purified antibody (concentration in sample 2 μg/ml, GAM FITC, red-filled histogram) binds specifically to surface phosphotyrosines in EGF treated A431 cells (upper panel), but not to the untreated A431 cells (lower panel). Level of non-specific binding was assessed using Mouse IgG1 isotype control purified antibody (MOPC-21) under same conditions (concentration in sample 2 μg/ml, GAM FITC, black-dashed histogram).

Quick Database Links

Gene Symbol

Phosphotyrosine

NCBI Gene ID

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Western Blot (IB, immunoblot)

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Flow Cytometry

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ICC

Immunocytochemistry

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