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RSK3 Antibody

SKU: orb1474470

Description

The RSK3 Antibody is suitable for IF, IHC, IP, WB. It is a Monoclonal, Unconjugated antibody which raised against A synthetic peptide of human RSK3 .Purification: The antibody was purified by immunogen affinity chromatography.

Images & Validation

Tested ApplicationsIF, IHC, IP, WB
Dilution rangeWB (1/500 - 1/1000), IH (1/50 - 1/100), IF/IC (1/50 - 1/100), IP (1/10 - 1/50)
ReactivityHamster, Human

Key Properties

Antibody TypePrimary Antibody
HostRabbit
ClonalityMonoclonal
ImmunogenA synthetic peptide of human RSK3
PurificationThe antibody was purified by immunogen affinity chromatography.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLiquid in 50mM Tris-Glycine (pH 7.4), 0.15M NaCl, 50% Glycerol, 0.01% Sodium azide and 0.05% rAlbumin.
DisclaimerFor research use only

Alternative Names

MAPKAPK1C; RSK3; Ribosomal protein S6 kinase alpha-2; S6K-alpha-2; 90 kDa ribosomal protein S6 kinase 2; p90-RSK 2; p90RSK2; MAP kinase-activated protein kinase 1c; MAPK-activated protein kinase 1c; MAPKAP kinase 1c; MAPKAPK-1c; Ribosomal S6 kinase 3; RSK-3; pp90RSK3

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RSK3 Antibody

Western blot analysis of RSK3 expression in CHOK1 (A), Hela (B) whole cell lysates. (Predicted band size: 83 kD; Observed band size: 90 kD)

RSK3 Antibody

Immunohistochemical analysis of RSK3 staining in human lung cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.89). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

RSK3 Antibody

Immunofluorescent analysis of RSK3 staining in U87MG cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark.

UniProt Details

No UniProt data available

NCBI Gene Details

No NCBI Gene data available

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
IHC
Immunohistochemistry
View Protocol
IF
Immunofluorescence
View Protocol
IP
Immunoprecipitation
View Protocol

RSK3 Antibody (orb1474470)

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Available Sizes

Select a size below

30 μl
¥ 1,690.00
50 μl
¥ 2,080.00
100 μl
¥ 3,250.00
200 μl
¥ 4,550.00
DispatchUsually dispatched within 5-10 working days
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