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SCIMP Antibody

SKU: orb154406

Description

Mouse monoclonal antibody to SCIMP which also known as Nvl, is a palmitoylated transmembrane adaptor protein expressed in professional antigen presenting cells, most prominently in the lymph nodes and spleen. It encodes a transmembrane adaptor protein that is expressed in antigen-presenting cells and is localized in the immunologic synapse.

Research Area

Epigenetics

Images & Validation

Tested ApplicationsFC, ICC, IP, WB
ReactivityHuman
Application Notes
Flow cytometry: Recommended dilution: 1-5 μg/ml. Intracellular staining.Western blotting: Recommended dilution: 1-2 μg/ml.

Key Properties

Antibody TypePrimary Antibody
ClonalityMonoclonal
IsotypeMouse IgG2a
Clone No.NVL-07
ImmunogenRecombinant intracellular part of human SCIMP
TargetSCIMP
PurificationPurified by protein-A affinity chromatography.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/PreservativesPhosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Concentration1 mg/ml
DisclaimerFor research use only

Alternative Names

NVL

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Quality Guarantee

Quality Guarantee

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SCIMP Antibody

Flow cytometry analysis (intracellular staining) of SCIMP in a population of HEK-293T-SCIMP transfectants using monoclonal antibody (clone NVL-07, purified).The staining pattern reflects heterogeneity in the cell population regarding transfection efficiency.

SCIMP Antibody

Western blotting analysis of human SCIMP using mouse monoclonal antibody NVL-07 on lysates of human SCIMP transfectants under reducing and non-reducing conditions. Nitrocellulose membrane was probed with 2 µg/ml of mouse monoclonal antibody followed by IRDye800-conjugated anti-mouse secondary antibody. SCIMP was detected around 17-22 kDa.

SCIMP Antibody

Flow cytometry intracellular staining pattern of human peripheral whole blood stained using anti-SCIMP (NVL-07) purified antibody (concentration in sample 1.67 µg/ml, GAM APC).

SCIMP Antibody

Separation of HeLa cells stained using anti-SCIMP (NVL-07) purified antibody (concentration in sample 5 µg/ml, GAM APC, red-filled) from HeLa cells unstained by primary antibody (GAM APC, black-dashed) in flow cytometry analysis (intracellular staining).

SCIMP Antibody

Separation of human monocytes (red-filled) from SCIMP negative lymphocytes (black-dashed) in flow cytometry analysis (intracellular staining) of human peripheral whole blood stained using anti-SCIMP (NVL-07) purified antibody (concentration in sample 1.67 µg/ml, GAM APC).

UniProt Details

No UniProt data available

NCBI Gene Details

No NCBI Gene data available

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol
FC
Flow Cytometry
View Protocol
ICC
Immunocytochemistry
View Protocol
IP
Immunoprecipitation
View Protocol

SCIMP Antibody (orb154406)

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0.1 mg
¥ 4,680.00
DispatchUsually dispatched within 5-10 working days weeks.
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