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Description
Research Area
Cancer Biology, Cell Biology, Disease Biomarkers, Metabolism Research
Images & Validation
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Item 1 of 2
| Tested Applications | ICC, IF, WB |
|---|---|
| Dilution Range | Western blot, 0.1-0.5μg/ml, Human Immunocytochemistry/Immunofluorescence, 5 μg/ml, Human |
| Reactivity | Human |
Related Conjugates & Formulations
−Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Polyclonal |
| Isotype | Rabbit IgG |
| Immunogen | E.coli-derived human SIRT1 recombinant protein (Position: R112-K311). Human SIRT1 shares 90% amino acid (aa) sequence identity with mouse SIRT1. |
| Target | NAD-dependent protein deacetylase sirtuin-1 |
| Molecular Weight | 120 kDa |
| Purification | Immunogen affinity purified. |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Lyophilized |
| Buffer/Preservatives | Each vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3. *This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required. |
| Concentration | Adding 0.2 ml of distilled water will yield a concentration of 500 μg/ml. |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−NAD-dependent protein deacetylase sirtuin-1; hSIRT1; 3.5.1.-; Regulatory protein SIR2 homolog 1; SIR2-like protein 1; hSIR2; SirtT1 75 kDa fragment; 75SirT1; SIRT1; SIR2L1
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Quick Database Links
Gene Symbol
NAD-dependent protein deacetylase sirtuin-1
UniProt
UniProt Details
− No UniProt data available
Protocol Information
WB
Western Blot (IB, immunoblot)
IF
Immunofluorescence
ICC
Immunocytochemistry
Available Sizes
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Free Secondary Antibody (20 ul)0/0
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