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Smad4 Rabbit Polyclonal Antibody

SKU: orb251580

Description

Anti-Smad4 Antibody. Tested in WB applications. This antibody reacts with Human, Mouse, Rat.

Research Area

Cancer Biology, Cardiovascular Research, Cell Biology, Epigenetics & Chromatin, Signal Transduction, Stem Cell & Developmental Biology

Images & Validation

Tested ApplicationsWB
Dilution RangeWestern blot, 0.1-0.5μg/ml, Human, Mouse, Rat
ReactivityHuman, Mouse, Rat

Key Properties

Antibody TypePrimary Antibody
HostRabbit
ClonalityPolyclonal
IsotypeRabbit IgG
ImmunogenA synthetic peptide corresponding to a sequence at the C-terminus of human Smad4, identical to the related mouse and rat sequences.
TargetMothers against decapentaplegic homolog 4
Molecular Weight60 kDa
PurificationImmunogen affinity purified.
ConjugationUnconjugated

Storage & Handling

StorageMaintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/AppearanceLyophilized
Buffer/PreservativesEach vial contains antibody formulated with stabilizing components, 0.9 mg NaCl, 0.2 mg Na2HPO4, and 0.05 mg NaN3. *This antibody is supplied in a stabilized formulation. Compatibility with conjugation reactions depends on the chemistry of the conjugation method used. For conjugation methods that are not compatible with the stabilizing components present in this formulation, a carrier-free antibody format is required.
ConcentrationAdding 0.2 ml of distilled water will yield a concentration of 500 μg/ml.
Expiration Date12 months from date of receipt.
DisclaimerFor research use only

Alternative Names

Mothers against decapentaplegic homolog 4; MAD homolog 4; Mothers against DPP homolog 4; Deletion target in pancreatic carcinoma 4; SMAD family member 4; SMAD 4; Smad4; hSMAD4; SMAD4; DPC4, MADH4

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Quality Guarantee

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

Smad4 Rabbit Polyclonal Antibody

Anti-SMAD4 Picoband antibody, Western blotting. All lanes: Anti SMAD4 at 0.5 ug/ml. Lane 1: Rat Brain Tissue Lysate at 50 ug. Lane 2: Mouse Brain Tissue Lysate at 50 ug. Lane 3: Rat Skeletal Muscle Tissue Lysate at 50 ug. Lane 4: Mouse Skeletal Muscle Tissue Lysate at 50 ug. Lane 5: U87 Whole Cell Lysate at 40 ug. Lane 6: Human Placenta Tissue Lysate at 50 ug. Lane 7: HT1080 Whole Cell Lysate at 40 ug. Lane 8: HELA Whole Cell Lysate at 40 ug. Lane 9: NEURO Whole Cell Lysate at 40 ug. Predicted bind size: 60 KD. Observed bind size: 60 KD.

UniProt Details

No UniProt data available

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Protocol Information

WB
Western Blot (IB, immunoblot)
View Protocol

Smad4 Rabbit Polyclonal Antibody (orb251580)

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Available Sizes

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100 μg
¥ 5,850.00
Free Secondary Antibody (20 ul)0/0

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DispatchUsually dispatched within 2-4 weeks
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