Synaptophysin Antibody

SKU: orb1474244

Description

The Synaptophysin Antibody is suitable for IHC, WB. It is a Monoclonal, Unconjugated antibody which raised against Purified recombinant fragment of SYP expressed in E. Coli. Purification: The antibody was purified by immunogen affinity chromatography.

Images & Validation

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Tested Applications	IHC, WB
Dilution range	WB (1/500 - 1/1000), IH (1/50 - 1/100)
Reactivity	Human, Mouse, Rat

Key Properties

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Antibody Type	Primary Antibody
Host	Mouse
Clonality	Monoclonal
Immunogen	Purified recombinant fragment of SYP expressed in E. Coli.
Purification	The antibody was purified by immunogen affinity chromatography.
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid in PBS containing 50% glycerol, 0.5% rAlbumin and 0.02% sodium azide, pH 7.3.
Disclaimer	For research use only

Alternative Names

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Synaptophysin; Major synaptic vesicle protein p38

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Western blot analysis of Synaptophysin expression in rat brain (A), rat hippocampus (B), Hela (C), NIH3T3 (D) whole cell lysates. (Predicted band size: 34 kD; Observed band size: 34 kD)

Immunohistochemical analysis of Synaptophysin staining in human tonsil formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.140). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

Quick Database Links

UniProt

Q62277, P08247, P07825

NCBI Gene ID

24804, 6855, 20977

UniProt Details

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No UniProt data available

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Western Blot (IB, immunoblot)

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IHC

Immunohistochemistry

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