TNFRSF10B Antibody

SKU: orb1174055

This product can be custom made

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Description

Anti-TNFRSF10B antibody(BFO335), Rabbit mAb

Research Area

Cell Biology

Images & Validation

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Item 1 of 3

Tested Applications	ELISA, FC
Dilution Range	ELISA 1/5000-10000\| Flow Cyt 1/100
Reactivity	Human

Key Properties

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Antibody Type	Recombinant Antibody
Host	Rabbit
Clonality	Monoclonal
Isotype	Rabbit IgG
Clone No.	BFO335
Target	TNFRSF10B
Purification	Purified from cell culture supernatant by affinity chromatography
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Form/Appearance	Liquid
Expiration Date	12 months from date of receipt.
Disclaimer	For research use only

Alternative Names

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TNFRSF10B;TRAILR2;TRAIL-R2;CD262;DR5;KILLER;TRICK2;ZTNFR9;TRICKB

Quality Guarantee

Explore bioreagents carefree to elevate your research. All our products are rigorously tested for performance. If a product does not perform as described on its datasheet, our scientific support team will provide expert troubleshooting, a prompt replacement, or a refund. For full details, please see our Terms & Conditions and Buying Guide. Contact us at [email protected].

ELISA plate pre-coated by 2 μg/ml (100 μL/well) Human TNFRSF10B protein, mFc tagged protein orb689451 can bind Rabbit anti-TNFRSF10B monoclonal antibody (clone: DM113) in a linear range of 0.6-90 ng/ml.

TNFRSF10B protein is highly expressed on the surface of Expi293 cell membrane. Flow cytometry analysis with Anti-TNFRSF10B on Expi293 cells transfected with human TNFRSF10B (Blue histogram) or Expi293 transfected with irrelevant protein (Red histogram), and Isotype antibody on Expi293 transfected with irrelevant protein (Orange histogram).

Flow cytometry analysis of antigen binding of rabbit anti-human TNFRSF10B mAb (orb1174055). (A) orb1174055 does not bind to TT cells that do not express TNFRSF10B. (B) A clear peak shift of orb1174055 was seen compared to the control when incubated with TNFRSF10B-expressing SNU-5 cells, indicating strong binding of orb1174055 to TNFRSF10B. Antibodies were incubated at 5 μg/mL.

Quick Database Links

Gene Symbol

TNFRSF10B

UniProt

O14763

UniProt Details

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No UniProt data available

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Protocol Information

Flow Cytometry

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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