TNFRSF7/CD27 Antibody

SKU: orb1806375

Description

Anti-TNFRSF7 / CD27 Reference Antibody is expressed from CHO. The heavy chain type is huIgG1, and the light chain type is hukappa. It has a predicted MW of 145.5 kDa.

Images & Validation

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Item 1 of 4

Tested Applications	ELISA, FA, FACS, In vivo
Reactivity	Human

Key Properties

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Clonality	Monoclonal
Target	TNFRSF7 / CD27
Source/Expression System	CHO
Molecular Weight	145 kDa
Purity	>95%
Purification	Protein A
Endotoxin	<0.001 EU/ug
Conjugation	Unconjugated

Storage & Handling

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Storage	Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Buffer/Preservatives	100 mM Proline/Acetic acid 20mM Arginine pH 5.0
Concentration	Batch dependent
Disclaimer	For research use only

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Anti-TNFRSF7 / CD27 Reference Antibody (varlilumab) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%.

The purity of Anti-TNFRSF7 / CD27 Reference Antibody (varlilumab)is more than 100% ;determined by SEC-HPLC.

Immobilized human CD27 FC at 2 μg/mL can bind Anti-TNFRSF7 / CD27 Reference Antibody (varlilumab);EC50=0.01912μg/mL.

HumanCD27 HEK293 cells were stained with Anti-TNFRSF7 / CD27 Reference Antibody (varlilumab) and negative control protein respectively; washed and then followed by PE and analyzed with FACS; EC142=0.095 ug/mL.

Quick Database Links

Gene Symbol

TNFRSF7 / CD27

UniProt

P26842

UniProt Details

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No UniProt data available

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Protocol Information

FACS

Fluorescence-Activated Cell Sorting (FC, Flow cytometry)

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ELISA

Enzyme-linked Immunosorbent Assay (EIA)

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