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TRIM25 Rabbit Monoclonal Antibody
SKU: orb548177
Description
Research Area
Cell Biology, Epigenetics & Chromatin, Protein Biochemistry, Signal Transduction
Images & Validation
−Item 1 of 1
| Tested Applications | FC, ICC, IF, IHC, IP, WB |
|---|---|
| Dilution Range | WB 1:1000-5000 IHC 1:50-200 ICC/IF 1:50-200 IP 1:50 FC 1:60 |
| Reactivity | Human, Mouse, Rat |
Key Properties
−| Antibody Type | Primary Antibody |
|---|---|
| Host | Rabbit |
| Clonality | Monoclonal |
| Isotype | Rabbit IgG |
| Clone No. | ADFG-20 |
| Immunogen | A synthesized peptide derived from human TRIM25 Functions as an ubiquitin E3 ligase and as an ISG15 E3 ligase. Involved in innate immune defense against viruses by mediating ubiquitination of DDX58. Mediates 'Lys-63'-linked polyubiquitination of the DDX58 N-terminal CARD-like region which is crucial for triggering the cytosolic signal transduction that leads to the production of interferons in response to viral infection. |
| Target | E3 ubiquitin/ISG15 ligase TRIM25 |
| Molecular Weight | 71 kDa |
| Purification | Affinity-chromatography |
| Conjugation | Unconjugated |
Storage & Handling
−| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
|---|---|
| Form/Appearance | Liquid |
| Buffer/Preservatives | Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol, 0.4-0.5mg/ml rAlbumin. |
| Concentration | 0.5mg/ml |
| Expiration Date | 12 months from date of receipt. |
| Disclaimer | For research use only |
Alternative Names
−Peptidyl-prolyl cis-trans isomerase B; PPIase B; 5.2.1.8; CYP-S1; Cyclophilin B; Rotamase B; S-cyclophilin; SCYLP; PPIB; CYPB
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Western blot analysis of TRIM25 expression in HeLa cell lysate using anti-TRIM25 antibody (orb548177). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TRIM25 antigen affinity purified polyclonal antibody (Catalog # orb548177) at 0.5 ug/mL overnight at 4°C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-Rabbit IgG IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # orb90444) with Tanon 5200 system. A specific band was detected for TRIM25.
Quick Database Links
Gene Symbol
E3 ubiquitin/ISG15 ligase TRIM25
UniProt
UniProt Details
− No UniProt data available
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Datasheet
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Protocol Information
WB
Western Blot (IB, immunoblot)
IHC
Immunohistochemistry
FC
Flow Cytometry
IF
Immunofluorescence
ICC
Immunocytochemistry
IP
Immunoprecipitation
TRIM25 Rabbit Monoclonal Antibody (orb548177)
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