Vimentin Antibody (PE)

SKU: orb44575

Description

Mouse monoclonal antibody against Vimentin conjugated to PE

Research Area

Epigenetics, Infectious Diseases, Neuroscience

Images & Validation

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Item 1 of 1

Tested Applications	FC
Reactivity	Human
Application Notes	Flow cytometry: Recommended dilution: 1-5 μg/ml. Intracellular staining.

Key Properties

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Antibody Type	Primary Antibody
Clonality	Monoclonal
Isotype	Mouse IgG1
Clone No.	VI-RE/1
Immunogen	Bacterially expressed full-length human vimentin
Target	Vimentin
Purification	Purified antibody is conjugated with R-phycoerythrin (PE) under optimum conditions. Unconjugated antibody and free fluorochrome are removed by size-exclusion chromatography.
Conjugation	PE

Storage & Handling

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Storage	Store at 2-8°C. Protect from prolonged exposure to light. Do not freeze.
Buffer/Preservatives	Stabilizing phosphate buffered saline (PBS), pH 7.4, 15 mM sodium azide
Concentration	0.1 mg/ml
Disclaimer	For research use only

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Flow cytometry analysis (intracellular staining) of Vimentin expression in LEP-19 human fibroblast cell line using anti-human Vimentin (VI-RE/1) PE. Overlay with Isotype mouse IgG1 control (PPV-06).

Quick Database Links

Gene Symbol

Vimentin

UniProt

P08670

NCBI Gene ID

7431

UniProt Details

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No UniProt data available

NCBI Gene Details

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No NCBI Gene data available

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Protocol Information

Flow Cytometry

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